货品编号： Bioss.bsm-52316R

品牌： Bioss

品名： Rabbit Anti-Bax antibody

规格： 100ul

产品编号bsm-52316R英文名称Bax中文名称Bax重组兔单克隆抗体别名apoptosis regulator BAX; Apoptosis regulator BAX cytoplasmic isoform beta; Apoptosis regulator BAX membrane isoform alpha; Bax isoform psi; BAX protein cytoplasmic isoform delta; Bax protein cytoplasmic isoform delta. antibody Bax protein cytoplasmic isoform gamma; Bax zeta; Bax-protein; Bcl-2-like protein 4; BCL2 associated X protein; BCL2L4; BAX_HUMAN; Bcl2-L-4.Specific References(1)|bsm-52316R has been referenced in 1 publications.[IF=]Shuang Huet al. MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1. Life Sci . 2020 Oct 1;258:118147.WB;Human.PubMed:32721464研究领域细胞生物信号转导细胞凋亡抗体来源Rabbit克隆类型Monoclonal克隆号3C3交叉反应Human,Mouse,Rat,产品应用WB=1:500-2000IHC-P=1:50-200IHC-F=1:50-200ICC=1:100（石蜡切片需做抗原修复）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分子量21kDa细胞定位细胞浆细胞膜性状Liquid浓度1mg/ml免疫原KLH conjugated synthetic peptide derived from human Bax亚型IgG纯化方法affinity purified by Protein A储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008].Function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.Subunit:Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Isoform Sigma interacts with BCL2A1 and BCL2L1 isoform Bcl-X(L). Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2.Subcellular Location:Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.Isoform Beta: Cytoplasm.Isoform Gamma: Cytoplasm.Isoform Delta: Cytoplasm (Potential).Tissue Specificity:Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.Similarity:Belongs to the Bcl-2 family.SWISS:Q07812Gene ID:581Database links:

Entrez Gene: 581Human

Entrez Gene: 12028Mouse

Entrez Gene: 24887Rat

Omim: 600040Human

SwissProt: Q07812Human

SwissProt: Q07813Mouse

SwissProt: Q63690Rat

Unigene: 624291Human

Unigene: 19904Mouse

Unigene: 10668Rat

Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品图片Sample:Testis (Mouse) Lysate at 40 ugTestis (Rat) Lysate at 40 ugSpleen (Mouse) Lysate at 40 ugSpleen (Rat) Lysate at 40 ugLung (Mouse) Lysate at 40 ugLung (Rat) Lysate at 40 ugMCF-7(Human) Cell Lysate at 30 ugHela(Human) Cell Lysate at 30 ugRaji(Human) Cell Lysate at 30 ugPrimary: Anti-Bax (bsm-52316R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 18'21 kDObserved band size: 21 kDSH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bax) monoclonal Antibody, Unconjugated (bsm-52316R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.