货品编号： Bioss.bs-3884R

品牌： Bioss

品名： Rabbit Anti-GPX4 antibody

规格： 100ul

产品编号bs-3884R英文名称GPX4中文名称谷胱甘肽过氧化酶4抗体别名Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase.研究领域肿瘤细胞生物免疫学信号转导新陈代谢线粒体抗体来源Rabbit克隆类型Polyclonal交叉反应Human,Mouse,Rat, (predicted: Pig,Cow,)产品应用WB=1:500-2000ELISA=1:5000-10000

not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.分子量22kDa细胞定位细胞浆线粒体性状Liquid浓度1mg/ml免疫原KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4:101-197/197亚型IgG纯化方法affinity purified by Protein A储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.PubMedPubMed产品介绍Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.Function:Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage.Subunit:Monomer. Has a tendency to form higher mass oligomers.Subcellular Location:Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm.Tissue Specificity:Present primarily in testis.Similarity:Belongs to the glutathione peroxidase family.SWISS:P36969Gene ID:2879Database links:

Entrez Gene: 2879Human

Entrez Gene: 625249Mouse

Entrez Gene: 29328Rat

Omim: 138322Human

SwissProt: P36969Human

SwissProt: O70325Mouse

SwissProt: P36970Rat

Unigene: 433951Human

Unigene: 359573Mouse

Unigene: 3647Rat

Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.谷胱甘肽过氧化物酶（GSH-Px）是机体内广泛存在的一种重要的过氧化物分解酶。硒是GSH-Px酶系的组成成分，它能催化GSH变为GSSG，使有毒的过氧化物还原成无毒的羟基化合物，同时促进H2O2的分解，从而保护细胞膜的结构及功能不受过氧化物的干扰及损害。GSH-Px的活性中心是硒半胱氨酸，其活力大小可以反映机体硒水平。GSH-Px酶系主要包括4种不同的GSH-Px，分别为胞浆GSH-Px、血浆GSH-Px、磷脂氢过氧化物GSH-Px及胃肠道专属性GSH-Px。胞浆GSH-Px由4个相同的分子量大小为22kDa的亚基构成四聚体，每个亚基含有1个分子硒半胱氨酸，广泛存在于机体内各个组织，以肝脏红细胞为最多。它的生理功能主要是催化GSH参与过氧化反应，清除在细胞呼吸代谢过程中产生的过氧化物和羟自由基，从而减轻细胞膜多不饱和脂肪酸的过氧化作用。产品图片Sample: Kidney (mouse) Lysate at 40 ugPrimary: Anti-GPX4 (bs-3884R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22kDObserved band size: 20 kDSample:Lane 1: Testis (Mouse) Tissue Lysate at 40 ugLane 2: Liver (Mouse) Tissue Lysate at 40 ugLane 3: Kidney (Mouse) Tissue Lysate at 40 ugLane 4: Heart (Mouse) Tissue Lysate at 40 ugLane 5: Testis (Rat) Tissue Lysate at 40 ugLane 6: Liver (Rat) Tissue Lysate at 40 ugLane 7: Kidney (Rat) Tissue Lysate at 40 ugLane 8: Heart (Rat) Tissue Lysate at 40 ugLane 9: U937 (Human) Cell Lysate at 30 ugLane 10: HepG2 (Human) Cell Lysate at 30 ugLane 11: MCF-7 (Human) Cell Lysate at 30 ugLane 12: Huvec (Human) Cell Lysate at 30 ugLane 13: 293T (Human) Cell Lysate at 30 ugPrimary: Anti-GPX4 (bs-3884R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 20-22 kDSample:Testis (Mouse) Lysate at 40 ugPrimary: Anti-GPX4 (bs-3884R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 22 kDObserved band size: 19 kD