货品编号： 博奥森.bs-5125R

品牌： 博奥森

品名： 卷曲蛋白FZD7抗体

规格： 50ul

产品介绍Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Frizzled family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. Frizzled 7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. Frizzled 7 expression has been reported in brain, gastrointestinal tract, heart, fetal kidney, fetal lung, placenta, skeletal muscle, and various cancers. ESTs have been isolated from a wide variety of normal and cancer libraries.Function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.Subunit:Interacts with MAGI3 and DVL1.Subcellular Location:Membrane; Multi-pass membrane protein.Tissue Specificity:High expression in adult skeletal muscle and fetal kidney, followed by fetal lung, adult heart, brain, and placenta. Specifically expressed in squamous cell esophageal carcinomas.Similarity:Belongs to the G-protein coupled receptor Fz/Smo family.Contains 1 FZ (frizzled) domain.SWISS:O75084Gene ID:8324Database links:

Entrez Gene: 8324Human

Entrez Gene: 374060Chicken

Entrez Gene: 524255Cow

Entrez Gene: 488478Dog

Entrez Gene: 14369Mouse

Entrez Gene: 301440Rat

GenBank: NP_003498.1Human

Omim: 603410Human

SwissProt: O57329Chicken

SwissProt: O75084Human

SwissProt: Q61090Mouse

Unigene: 173859Human

Unigene: 297906Mouse

Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.产品图片Sample:HepG2(Human) Cell Lysate at 30 ugMuscle (Mouse) Lysate at 40 ugMuscle (Rat) Lysate at 40 ugPrimary: Anti-Frizzled 7 (bs-5125R) at 1/2000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60 kDObserved band size: 60 kDSample:Muscle (Mouse) Lysate at 40 ugPrimary: Anti-Frizzled 7 (bs-5125R) at 1/1000 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 60 kDObserved band size: 60 kDBlank control: A431.Primary Antibody (green line): Rabbit Anti-Frizzled 7 antibody (bs-5125R)Dilution: 3μg /10^6 cells;Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody: Goat anti-rabbit IgG-AF647Dilution: 3μg /test.ProtocolThe cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.