bioss.bs-2332R 二脂酰甘油酰基转移酶抗体 50ulBioss-耗材-金山科研平台 Thermo Fisher官网Nunc、QSP、Nalgene、Invitrogen、Gibco耗材试剂授权一级代理

bioss.bs-2332R 二脂酰甘油酰基转移酶抗体 50ulBioss

货品编号： bioss.bs-2332R

品牌： bioss

品名：二脂酰甘油酰基转移酶抗体

规格： 50ul

产品介绍

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.Function:Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.Subunit:Homodimer or homotetramer.Subcellular Location:Endoplasmic reticulum membrane; Multi-pass membrane protein.Similarity:Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.SWISS:O75907Gene ID:8694Database links:

Entrez Gene: 482093Dog

Entrez Gene: 8694Human

Entrez Gene: 13350Mouse

Entrez Gene: 84497Rat

Omim: 604900Human

SwissProt: O75907Human

SwissProt: Q9Z2A7Mouse

SwissProt: Q9ERM3Rat

Unigene: 521954Human

Unigene: 22633Mouse

Unigene: 252Rat

Important Note:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Sample: Hela (human)cell Lysate at 40 ugPrimary: Anti- DGAT1 (bs-2332R)at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55kDObserved band size: 55 kDSample:Lane 1: Mouse Adipose tissue lysatesLane 2: Mouse Adrenal gland tissue lysatesLane 3: Rat Adipose tissue lysatesLane 4: Rat Stomach tissue lysatesLane 5: Rat Testis tissue lysatesLane 6: Human HL60 cell lysatesLane 7: Human HepG2 cell lysatesPrimary: Anti-DGAT1 (bs-2332R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 55 kDObserved band size: 60 kDParaformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DGAT1) Polyclonal Antibody, Unconjugated (bs-2332R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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bioss.bs-2332R 二脂酰甘油酰基转移酶抗体 50ulBioss

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