产品英文名称：CHO-CM HCP ELISA kit

产品中文名称：CHO-CM HCP ELISA 试剂盒

CHO-CM HCP ELISA kit 的应用

本试剂盒是用于确定在CHO细胞重组表达产品中CHO宿主细胞蛋白（HCPS）的存在。本试剂盒适合使用的抗体通常发现在无蛋白的条件培养介质相生成。该试剂盒是用于研究和制造使用，不用于人类或动物的诊断应用。

CHO-CM HCP ELISA 试剂盒的英文描述

This assay is intended for use on pharmaceutical products secreted into protein-free CHO growth media. The antibody is raised against, affinity purified, and calibrated with HCPs typically found in CHO protein-free conditioned media. Special procedures were utilized in the generation of these antibodies to insure that low molecular weight and less immunogenic contaminants as well as high molecular weight components would be represented. Our assay validation has determined that the LOD for this kit is <200pg/mL.

CHO-CM HCP ELISA kit 的使用步骤规则

The CHO HCP assay is a sequential, two-site immunoenzymetric assay also termed ELISA. Samples potentially containing CHO proteins are reacted in microtiter strips coated with an affinity purified capture antibody. After a wash step, a horseradish peroxidase enzyme labeled anti-CHO antibody is reacted sequentially forming a sandwich complex of solid phase antibody-CHO protein-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of CHO proteins present.

CHO-CM HCP ELISA kit 的保存和稳定性

* All reagents should be stored at 2C to 8C for stability until the expiration date printed.

*Reconstituted wash solution is stable until the expiration date of the kit.

CHO-CM HCP ELISA kit 的使用注意事项

*只用于研究或制造。

*停止反应试剂是0.5M H2SO4。避免接触眼睛，皮肤，和服装。在用本试剂盒的试剂浓度，其他没有被认为是有害的。

*本试剂盒只能由合格的技术人员使用。