产品英文名称：HEK 293 HCP ELISA kit

产品中文名称：HEK 293 细胞 ELISA 试剂盒

HEK 293 HCP ELISA kit 的应用

本试剂盒是用于确定在HEK 293细胞表达产品中宿主细胞蛋白污染的存在。该试剂盒是用于研究和制造使用，不用于人类或动物的诊断应用。这是HEK HCP检测第二代ELISA试剂盒。这种新的试剂盒，取代原来的F150 ELISA试剂盒，不再是市售。第二代测定使用一个更广泛的反应性抗体。这样的试剂盒可以产生与使用旧F150试剂盒不同的HEK HCP污染水平的报告。

HEK 293 细胞 ELISA 试剂盒的英文描述

Cygnus is pleased to announce the re-introduction of HEK 293 Host Cell Protein ELISA kit. This 2nd Generation kit utilizes antibodies derived from the same pool of goat anti-HEK antiserum used in the 1st Generation kit, however this antiserum has now been affinity purified with new and a more represenatitve preparation of HCPs typically found in virus, vaccine and recombinant protein products.

HEK 293 HCP ELISA kit 的使用步骤规则

The HEK assay is a two-site immunoenzymetric assay. Samples containing HEK HCPs are reacted simultaneously with a horseradish peroxidase (HRP) enzyme labeled anti-HEK antibody (goat polyclonal) in microtiter strips coated with an affinity purified capture anti-HEK antibody. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-HCP-enzyme labeled antibody. The microtiter strips are washed to remove any unbound reactants. The substrate, tetramethyl benzidine (TMB) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and is directly proportional to the concentration of HEK HCPs present.

HEK 293 HCP ELISA kit 的保存和稳定性

* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.

* The substrate reagent should not be used if its stopped absorbance at 450nm is greater than 0.1.

* Reconstituted wash solution is stable until the expiration date of the kit.

HEK 293 HCP ELISA kit 的使用注意事项

*只用于研究或制造。

*停止反应试剂是0.5N H2SO4。避免接触眼睛，皮肤，和服装。在用本试剂盒的试剂浓度，其他没有被认为是有害的。

*本试剂盒只能由合格的技术人员使用。