英文名称：Recombinant Human Erythropoietin-alpha Fc/Chimera

中文简称：【EPO a Fc】重组人红细胞生成素-α受体/嵌合体

Recombinant Human Erythropoietin-alpha Fc/Chimera 【EPO a Fc】重组人红细胞生成素-α受体/嵌合体 细胞因子描述：

红细胞生成素（erythropoietin，EPO）对红细胞生成的特异性刺激作用的细胞因子。红细胞生成素是一种糖蛋白，在胎儿体内由肾脏及肝脏产生，而在成人体内主要由肾脏产生。目前已知人红细胞生成素有两种存在形式，即人EPO-α及人EPO-β，二者氨基酸组成及顺序相同，都含有165个氨基酸残基。分子量、等电点及生物活性也都类似，差别在于二者的糖型组成不同，EPO-α含有较多的N-乙酰氨基葡萄糖和N-乙酰神经氨酸，总的含糖量也较EPO-β高。红细胞生成素-α是调节红细胞增殖、成熟和分化的一种造血生长因子。

人红细胞生成素-α Fc/嵌合体重组蛋白是在CHO中国仓鼠卵巢细胞中重组表达的，是由两条糖基化多肽链组成的二聚物，其中两条成熟的人EPO分子连接到人IgG1的Fc部位。由于发生了糖基化，该重组嵌合体蛋白在非变性SDS-PAGE中的显示分子量为140kDa。

CHO中国仓鼠卵巢细胞中重组表达

Recombinant Human Erythropoietin-alpha Fc/Chimera 【EPO a Fc】重组人红细胞生成素-α受体/嵌合体 细胞因子实验须知：

溶解：提供的人红细胞生成素-α受体/嵌合体(EPO a Fc)产品为过滤灭菌的白色冻干粉末；该冻干粉末中每毫克人红细胞生成素-α受体/嵌合体是从1x PBS（pH 7.4）溶液中过滤并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人红细胞生成素-α受体/嵌合体(EPO a Fc)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Erythropoietin-alpha Fc/Chimera 【EPO a Fc】重组人红细胞生成素-α受体/嵌合体 细胞因子英文描述：

Introduction

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Description

Erythropoietin-alpha Fc-Chimera Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a dimeric, glycosilated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.

Source

Chinese Hamster Ovary Cells(CHO).

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Each mg of lyophilized powder contains 1x PBS pH-7.4.

Solubility

It is recommended to reconstitute the lyophilized Erythropoietin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity

Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Biological Activity

The ED50 as determined by the dose-dependent stimulation of human megakaryoblastic leukemia cells is less than 2.0 ng/ml, corresponding to a Specific Activity of 5.0 x 105 IU/mg.

Usage

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.