英文名称：Recombinant Human Brain-Derived Neurotrophic Factor

中文简写：【BDNF】重组人脑源性神经营养因子

Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重组人脑源性神经营养因子 细胞因子描述：

脑源性神经营养因子（BDNF）最初从猪脑纯化小分子碱性蛋白质，是神经营养因子家族一个成员，为一种小碱性蛋白质。与神经生长因子不同，脑源性神经营养因子主要在中枢神经系统，维持神经脊和外胚层基板感觉神经元，这些神经元对神经生长因子无反应。脑源性神经营养因子在神经元存活、分化及突触可塑性调节中起重要作用，参与了大脑学习记忆过程。BDNF生物学作用主要通过其受体TrkB介导。

人脑源性神经营养因子重组蛋白是在大肠杆菌中重组表达的，是由两条非糖基化多肽链组成的同源二聚体，包含有2 x 119个氨基酸，总分子量为26，984Da。该人脑源性神经营养因子重组蛋白最终由标准的色谱技术纯化出。

来源宿主-大肠杆菌中重组表达

Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重组人脑源性神经营养因子细胞因子实验须知：

溶解：提供的人脑源性神经营养因子(BDNF)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含人脑源性神经营养因子(BDNF)的20mM 柠檬酸钠缓冲液（pH 5）中经透析并冻干产生的。我们建议在无菌的18MΩ-cm水中溶解，使其浓度不低于100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人脑源性神经营养因子(BDNF)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重组人脑源性神经营养因子 细胞因子英文描述：

Synonyms

Brain-Derived Neurotrophic Factor, BDNF, MGC34632.

Introduction

BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Description

Brain-Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton.

BDNF Human Recombinant is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

The protein was lyophilized WITHOUT ANY ADDITIVES.

Solubility

It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity

BDNF is greater than 97.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids of BDNF was determined and found to be Met-His-Ser-Asp-Pro.

Biological Activity

The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg.

Protein content

BDNF quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.