英文名称：Recombinant Human Interleukin-3

中文简称：【IL 3 】重组人白介素-3

Recombinant Human Interleukin-3 【IL 3 】重组人白介素-3 细胞因子产品描述：

人白介素-3重组蛋白促进细胞因子是一个强有力的增长。这种细胞因子能够支持广泛的造血细胞的增殖。它是参与多种细胞活动,如细胞生长、分化和凋亡。这种细胞因子也具有神经营养活性,而且它可能与神经系统疾病有关。

人白介素-3重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有133个氨基酸，分子量为15000Da。该人白介素-3重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Interleukin-3 【IL 3 】重组人白介素-3 细胞因子产品实验须知：

溶解：提供的人白介素-3(IL 3)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人白介素-3(IL 3)的0.3 mg/ml NaHCO3 溶液中冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人白介素-3(IL 3 )冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装后置于-18℃冻存。请避免反复冻融。

Recombinant Human Interleukin-3 【IL 3 】重组人白介素-3 细胞因子产品英文描述：

Synonyms

MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Introduction

IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

Description Interleukin-3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 133 amino acids and having a molecular mass of 15000 Dalton. The IL-3 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 0.3 mg/ml of NaHCO3.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 98.0% as determined by:

(a) Analysis by SEC-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Thr-Gln.

Biological Activity The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.

Protein content Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard.