英文名称：Recombinant Human Interleukin-6

中文简称：【IL 6】重组人白介素-6

Recombinant Human Interleukin-6 【IL 6】重组人白介素-6 细胞因子产品描述：

人白介素-6重组蛋白是一种与多种细胞因子的生物学功能:它扮演一个重要角色在最后白成ig-secreting细胞分化,诱导骨髓瘤和浆细胞瘤增长,导致神经细胞分化,在肝细胞诱发急性期反应物。

人白介素-6重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有184个氨基酸，分子量为21000Da。该人白介素-6重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达。

Recombinant Human Interleukin-6 【IL 6】重组人白介素-6细胞因子产品实验须知：

溶解：提供的人白介素-6(IL 6)产品为经0.2um孔径过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人白介素-6(IL 6)的PBS，pH 7.4溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：人白介素-6(IL 6)在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Interleukin-6 【IL 6】重组人白介素-6 细胞因子产品英文描述：

Synonyms

IFN-b2, B cell differentiation factor, BCDF, BSF-2, HPGF, HSF, MGI-2, B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, CTL differentiation factor, CDF, IL-6, HGF.

Introduction

Il-6 is a cytokine with a wide variety of biological functions: it plays an essential role in the final differentiation of b-cells into ig-secreting cells, it induces myeloma and plasmacytoma growth, it induces nerve cells differentiation, in hepatocytes it induces acute phase reactants.

Description

Interleukin-6 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21000 Dalton.

The IL6 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity

Greater than 97.0% as determined by

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Pro-Val-Pro-Pro.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is less than 0.1 ng/ml, corresponding to the specific activity of 1.0 x 10,000,000 Units per mg.

Protein content

Protein quantitation was carried out by two independent methods

1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.