英文名称：Recombinant Human Bone Morphogenetic protein-2

中文简称：【BMP 2】重组人骨形态发生蛋白-2

Recombinant Human Bone Morphogenetic protein-2 【BMP 2】重组人骨形态发生蛋白-2 细胞因子产品描述：

骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2) 是转化生长因子β超家族成员之一，具有诱导未分化间充质干细胞向成软骨细胞和成骨细胞定向分化与增殖能力，促进成骨细胞分化成熟，参与骨和软骨生长发育及其重建过程，进而加速骨缺损修复。BMP-2在体内以前体形式合成，经蛋白酶切去除信号肽和前肽，得到由114个氨基酸残基组成成熟肽。成熟肽通过7对二硫键将其保守结构正确折叠。成熟肽同源或异源二聚体才具有生物活性。

人骨形态发生蛋白-2重组蛋白是在大肠杆菌中重组表达的，是由两条非糖基化多肽链组成的同源二聚体，包含有115个氨基酸，分子量为26018kDa。该人骨形态发生蛋白-2重组蛋白最终由标准的色谱技术纯化出。

来源宿主---大肠杆菌中重组表达

Recombinant Human Bone Morphogenetic protein-2 【BMP 2】重组人骨形态发生蛋白-2 细胞因子产品使用须知：

溶解：提供的人骨形态发生蛋白-2(BMP 2)产品为过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml人骨形态发生蛋白-2(BMP 2)的20mM NaAc（pH 4）溶液中过滤灭菌并冻干产生的。我们建议加无菌的20mM AcOH（乙酸）以配制工作储存液到不低于100ug/ml，后继实验过程 中可根据实验要求进一步稀释。

保存：人骨形态发生蛋白-2(BMP 2)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。

Recombinant Human Bone Morphogenetic protein-2 【BMP 2】重组人骨形态发生蛋白-2 细胞因子产品英文描述：

Synonyms

BMP-2, BMP2A.

Introduction

BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.

Description

Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 2x115 amino acids and having a molecular mass of 26kDa.

The BMP-2 is purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearance （外观）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

BMP2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.

Solubility （溶解性）

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20mM AcOH (acetic Acid) not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability（稳定性）

Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity（纯度）

Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.

Biological Activity The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 µg/ml.

Protein content （蛋白质含量）

Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.