英文名称：Recombinant Mouse Interferon-gamma

中文简称：【mIFN g】重组小鼠干扰素-γ

Recombinant Mouse Interferon-gamma 【mIFN g】重组小鼠干扰素-γ 细胞因子产品描述：

干扰素（interferon,IFN）是细胞对相关刺激反应时所产生的细胞信号蛋白质，是细胞因子超家族中一个糖蛋白类同源细胞因子家族，称为IFN家族。已鉴定出该家族中的3个主要成员：IFN-α、IFN-β和IFN-γ。其中干扰素-γ(Interferon-gamma，IFN-γ)是一种具有抗病毒、抗肿瘤和免疫调节作用的细胞因子，主要由活化的T细胞和NK细胞产生，在机体免疫系统中发挥重要作用。IFN-γ是一类分泌型糖蛋白，由143个氨基酸组成，相对分子量是16924。而重组人干扰素-γ相对分子量在14000-16000之间，这是由于重组IFN-γ在C末端有氨基酸丢失现象。

小鼠干扰素-γ重组蛋白是在大肠杆菌中重组表达的，是一条单链、非糖基化的多肽链，包含有134个氨基酸，分子量为15601Da。该小鼠干扰素-γ重组蛋白最终由标准的色谱技术纯化出。

来源宿主—大肠杆菌中重组表达

Synonyms

Immune Interferon, type II interferon, T cell interferon, MAF, IFNG, IFG, IFI, IFN-gamma.

Introduction

IFN-gamma produced by lymphocytes activated by specific antigens or mitogens.

IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Description

Interferon-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.

The IFN-gamma is purified by proprietary chromatographic techniques.

Source （来源）

Escherichia Coli.

Physical Appearance （物理特征）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2µm filtered concentrated (1mg/ml) solution in PBS, pH 7.4 and 5% trehalose.

Solubility

It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability （稳定性）

Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity （纯度）

Greater than 95.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Analysis by SDS-PAGE.

Amino acid sequence （氨基酸序列）

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.

Biological Activity （生物活性）

The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.

Recombinant Mouse Interferon-gamma 【mIFN g】重组小鼠干扰素-γ 细胞因子产品使用须知：

溶解：提供的小鼠干扰素-γ(mIFN g)产品为经0.2um孔径过滤灭菌的白色冻干粉末；该冻干粉末是从含1mg/ml小鼠干扰素-γ(mIFN g)的PBS（pH 7.4）溶液中过滤灭菌并冻干产生的。我们建议加无菌的18MΩ-cm水以配制工作储存液到100ug/ml，后继实验过程中可根据实验要求进一步稀释。

保存：小鼠干扰素-γ(mIFN g)冻干粉末在室温可保存3周，或置于-18℃冻存用于长期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延长保存时间，建议分装（加载体蛋白如0.1% HSA或 BSA）后置于-18℃冻存。请避免反复冻融。