产品英文名称:Recombinant Human GM‑CSF GMP
产品中文名称:重组人GM-CSF
GM-CSF的作用:
GM-CSF能刺激粒细胞及巨噬细胞等白细胞的增殖、分化及活化作用,从而增强造血功能。它也能增强中性粒细胞,嗜曙红细胞及单核细胞的多种功能。它还能促使效应细胞增强如吞噬细菌及消灭癌细胞等免疫活动。
R&D 重组人GM-CSF 人粒细胞-巨噬细胞集落刺激因子 Recombinant Human GM‑CSF GMP英文描述:
Source: E. coli-derived, Ala25-Glu144
Accession #: P04141
N-terminal Sequence Analysis: Ala18-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr
Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level: < 0.1 EU per 1 µg of the protein by the LAL method.
Predicted Molecular Mass: 14.5 kDa
SDS-PAGE: 14 kDa, reducing conditions
Activity: Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is typically 6-30 pg/mL.
Formulation: Lyophilized from a 0.2 µm filtered solution in PBS. See Certificate of Analysis for details.
roduced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.
Reconstitution: Reconstitute at 100-200 µg/mL in sterile PBS.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
·12 months, -20 to -70 °C as supplied.
·1 month, 2 to 8 °C under sterile conditions after reconstitution.
·3 months, -20 to -70 °C under sterile conditions after reconstitution.
R&D 重组人GM-CSF 人粒细胞-巨噬细胞集落刺激因子 Recombinant Human GM‑CSF GMP实验数据:
1 µg/lane of GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) stimulates proliferation of TF-1 human erythroleukemic cell line. The ED50 is typically 6-30 pg/mL.
MALDI-TOF analysis of GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP). The major peak corresponds to the calculated molecular mass, 14468 Da. The minor peak at 14673 Da is a matrix-associated artifact of the MALDI-TOF.
R&D 重组人GM-CSF 人粒细胞-巨噬细胞集落刺激因子 Recombinant Human GM‑CSF GMP系列产品订购新:
Recombinant Human GM-CSF GMP, CF
|
215-GMP-01M |
1 mg |
Please Inquire |
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Recombinant Human GM-CSF GMP, CF
|
215-GMP-010 |
10 µg |
Please Inquire |
|
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Recombinant Human GM-CSF GMP, CF
|
215-GMP-050 |
50 µg |
Please Inquire |
|
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