产品英文名称：Recombinant Human IL‑4 GMP

产品中文名称：重组人IL-4GMP

R&D 重组人IL-4GMP Recombinant Human IL‑4 GMP产品英文介绍：

Source: E. coli-derived, His25-Ser153, with an N-terminal Met

Accession #: P05112

N-terminal Sequence Analysis: Met-His-Lys-(Cys)-Asp-Ile-Thr-Leu-Gln-Glu

Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level: < 0.1 EU per 1 µg of the protein by the LAL method.

Predicted Molecular Mass: 15.1 kDa

SDS-PAGE: 14 kDa, reducing conditions

Activity: Measured in a cell proliferation assay using TF 1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.

The ED50 for this effect is typically 0.05-0.2 ng/mL.

Formulation: Lyophilized from a 0.2 µm filtered solution in PBS. See Certificate of Analysis for details.

Produced using non-animal reagents in an animal-free laboratory.

Manufactured and tested under current Good Manufacturing Practice (GMP) guidelines.

Reconstitution: Reconstitute at 100-200 µg/mL in sterile PBS.

Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

·12 months, -20 to -70 °C as supplied.

·1 month, 2 to 8 °C under sterile conditions after reconstitution.

·3 months, -20 to -70 °C under sterile conditions after reconstitution.

R&D 重组人IL-4GMP Recombinant Human IL‑4 GMP产品数据图像：

1 µg/lane of GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP) stimulates proliferation of TF-1 human erythroleukemic cell line. The ED₅₀ is typically 0.05-0.2 ng/mL.

MALDI-TOF analysis of GMP-grade Recombinant Human IL-4 (Catalog # 204-GMP). The major peak corresponds to the calculated molecular mass, 15085 Da. The minor peak at 15297 Da is a matrix-associated artifact of the MALDI-TOF.

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