产品英文名称：mTeSR™1

产品中文名称：iPSC/ESC无血清培养基

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1（产品号 #05850）/mTeSR™2 （产品号#05860）产品简介：

STEMCELL Technologies 研发的mTeSR™1（产品号 #05850）和 mTeSR™2（产品号#05860），作为无饲养层细胞条件下维持 hPSC 的标准化培养基。它们的成分基于 Ludwig等公布的研究成果，是完全的、成分确定的无血清培养基，其生产得到 WiCell Research Institute 的授权。mTeSR?1含牛血清白蛋白来源，用于支持长期、无饲养层细胞条件下培养 hPSC 以及在无饲养层细胞条件下衍生 hiPSC。TeSR?2则是不含动物蛋白的配方，同样支持长期、无饲养层细胞条件下培养 hPSC。两者均不需要进一步添加生长因子。它们旨在维持和扩增未分化的hPSC，并与作为基质的 BD Matrigel? hESC-qualified Matrix（BD 产品号#354277）配合使用。STEMCELL Technologies 预先对每一批次的 BD Matrigel?的质量进行检测，确保均一性、可重复性和可靠性。若要获得完全不含动物蛋白的培养体系，TeSR?2 可与Vitronectin第替代基质配合使用。

在 mTeSR™1 和 TeSR™2 中培养的 hPSC：

· 表型均匀且核型正常

· 表达高水平的多能性标记如：Oct-3/4、SSEA-3、SSEA-4、TRA-1-60、TRA-1-81

· 表达多能性所需的基因：Oct-3/4、nanog

· 形成包含内、中、外胚层谱系衍生物的畸胎瘤

· 可在体外分化为功能成熟的细胞类型（如造血细胞）

· 从基于饲养层细胞的培养物转化时，不需要驯化培养期（即无细胞低产期）

使用 mTeSR™1 和 TeSR™2 的优点包括：

· 去除不确定的培养基成分和消除与饲养层细胞和条件培养基有关的固有可变性，使 hPSC 培养的条件保持一致性

· 标准化培养方法，从而提高数据的可重复性

· 省去制备饲养层细胞或条件培养基的程序，节省了时间和劳力

· 完全的培养基配方，无需添加额外的生长因子或其他添加物

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1（产品号 #05850）/mTeSR™2 （产品号#05860）相关产品订购信息：

Product Name Description Catalog # Size Price Quantity

mTeSR?1 Defined, feeder-free maintenance medium for human ESCs and iPSCs 05850 500 mL Kit Request pricing

mTeSR?1 Defined, feeder-free maintenance medium for hESCs and hiPSCs, 1 L kit 05857 1 L Kit Request pricing

mTeSR?1 Defined, feeder-free maintenance medium for hESCs and hiPSCs, 10 pack 05870 10 Kits Request pricing

mTeSR?1 Defined, feeder-free maintenance medium for hESCs and hiPSCs, 25 pack 05875 25 Kits Request pricing

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR?1（产品号 #05850）/TeSR?2 （产品号#05860）英文描述：

mTeSR™1 is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain hundreds of ES cell and iPS cell lines in over 40 countries, and has supported top pluripotent stem cell publications and researchers.

mTeSR™1 is a highly specialized, serum-free and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.

STEMCELL iPSC/ESC无血清培养基 化学成分确定的 mTeSR™1（产品号 #05850）/mTeSR™2 （产品号#05860）其他相关产品订购信息：

Product NameDescription Catalog #

TeSR™2 Animal protein-free, defined, feeder-independent medium for maintenance of undifferentiated human ES and iPS cells 05860

AggreWell™ Simple and Standardized Production of Embryoid Bodies 27845

mFreSR™ Defined, Serum-Free Cryopreservation Medium for Human ES cells and iPS cells 05854

CryoStor® CS10 Animal Component-Free, Defined Cryopreservation Medium 07930

EasySep™ hESC-Derived CD34 Positive Selection Kit Immunomagnetic Positive Selection Kit 18167

Mouse IgG (H+L) Antibody, FITC-Conjugated Goat Polyclonal Antibody to Mouse IgG (H+L) – FITC Conjugated 10210

IgM Antibody, FITC-Conjugated Goat Polyclonal Antibody to Mouse IgM – FITC Conjugated 10211

IgM Antibody, APC-Conjugated Goat Polyclonal Antibody to Rat IgM – APC Conjugated 10215

ACCUTASE™ ACCUTASE™ Cell Detachment Solution 07920

Dispase (1 U/mL) Dispase 1 U/mL 07923

STEMdiff™ APEL™ Medium Serum-Free, Animal Component-Free Medium for Differentiation of Human ES and iPS cells to Multiple Lineage Types 05210

StemAdhere™ Defined Matrix for hPSC For maintenance of human ES and iPS cells in combination with mTeSR™1 or TeSR™2 07160

Anti-Sox-2 Antibody Rabbit Polyclonal Antibody Against Human Sox-2 60055

Anti-Mouse SSEA-1 (CD15) Antibody, Clone MC-480 Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-1 (CD15) 60060

Anti-Mouse SSEA-3 Antibody, Clone MC-631 Rat Monoclonal Antibody Against Human, Mouse, Rat SSEA-3 60061

Anti-Human SSEA-4 Antibody, Clone MC-813-70 Mouse Monoclonal Antibody Against Human, Mouse, Rat SSEA-4 60062

Anti-Human TRA-1-60 Antibody, Clone TRA-1-60R Mouse Monoclonal Antibody Against Human, Rhesus, Rabbit TRA-1-60 (Podocalyxin) 60064

Anti-Human TRA-1-81 Antibody, Clone TRA-1-81 Mouse Monoclonal Antibody Against Human, Rat, Rhesus TRA-1-81 60065

Anti-Human TRA-2-49 Antibody, Clone TRA-2-49/6E Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-49 (Tissue Non-Specific Alkaline Phosphatase) 60066

Anti-Human TRA-2-54 Antibody, Clone TRA-2-54/2J Mouse Monoclonal Antibody Against Human, Chimpanzee, Gibbon TRA-2-54 (Tissue Non-Specific Alkaline Phosphatase) 60067

Mouse IgM, kappa Isotype Control Antibody, Clone MM-30 Mouse Monoclonal IgM, kappa Isotype Control Antibody 60069

Gentle Cell Dissociation Reagent Enzyme-Free Cell Dissociation Reagent 07174

STEMdiff™ Definitive Endoderm Kit Fully Defined Animal Component-Free Medium for the Differentiation of Human ES and iPS Cells to Definitive Endoderm. 05110

Oct-3/4 Antibody, Clone 40 Mouse Monoclonal Antibody to Oct-3/4 01550

Vitronectin XF™ Chemically Defined, Xeno-Free Cell Attachment Factor that Supports the Growth and Differentiation of Human Pluripotent Stem Cells Under Serum-Free, Feeder-Free Conditions 07180

STEMdiff™ Neural Induction Medium Defined, Serum-Free Induction Medium for Neural Differentiation of Human ES and iPS Cells