β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.
The BioGems Murine β-NGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure β-NGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for β-NGF. It contains NS0-expressed recombinant β-NGF. The detection antibody is a biotinylated goat polyclonal antibody specific for β-NGF. The kit is analytically validated with ready to use reagents.
β-NGF是与BDNF，NT-3和NT-4蛋白结构相关的神经营养因子。这些蛋白属于可能与稳定二聚体结构相关的半胱氨酸结家族生长因子。 β-NGF是通过其受体β-NGFR传导信号的强效神经营养因子，并且在感觉和交感神经系统的发育和保存中起关键作用。 β-NGF还充当B淋巴细胞的生长和分化因子，并增强B细胞存活。
BioGems鼠β-NGF预包被ELISA（酶联免疫吸附试验）试剂盒采用固相免疫测定试剂盒，专门设计用于检测β-NGF，其中96孔微孔板预包被β-NGF特异小鼠单克隆抗体NGF。它含有NS0表达的重组β-NGF。检测抗体是对β-NGF特异的生物素化山羊多克隆抗体。该试剂盒经过分析验证，是即用型试剂。
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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|
Sensitivity:
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<1pg/ml
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|
Range:
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31.2pg/ml-2000pg/ml
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|
Specificity:
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Natural and recombinant Murine β-NGF
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|
Uniprot:
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P01139
|
|
Interference:
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No detectable cross-reactivity with other relevant proteins
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|
Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components组份
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Description
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Quantity
|
Volume
|
|
Anti-Murine β-NGF Pre-coated 96-well strip microplate
|
1
|
12 strips of 8 wells
|
|
Murine β-NGF Standard
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2
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10 ng vial
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Murine β-NGF Biotinylated antibody (100x)
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1
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130 µL
|
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Avidin-Biotin-Peroxidase Complex (100x)
|
1
|
130 µL
|
|
Sample Diluent
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1
|
30mL
|
|
Antibody Diluent
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1
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12mL
|
|
Avidin-Biotin-Peroxidase Diluent
|
1
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12mL
|
|
Wash Buffer Concentrate (Powder for 1000mL)
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1
|
Pack
|
|
Color Developing Reagent
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1
|
10mL
|
|
Stop Solution
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1
|
10mL
|
|
Plate Sealers
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4
|
Each
|
Reproducibility可重复性
|
|
Intra-Assay
|
Inter-Assay
|
|
Sample ID
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n=
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean Measured Concentration (pg/mL)
|
136
|
727
|
1326
|
153
|
839
|
1645
|
|
Standard Deviation
|
5.3
|
41.44
|
83.54
|
6.58
|
53.7
|
121.73
|
|
Consistency (%CV)
|
3.9
|
5.7
|
6.3
|
4.3
|
6.4
|
7.4
|
窗体底端
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