Catalog Number :BGK9QYH9
LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTβR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NF-κB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells.
The BioGems Murine Light Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Light with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Light. It contains NS0-expressed recombinant Light. The detection antibody is a biotinylated goat polyclonal antibody specific for Light. The kit is analytically validated with ready to use reagents.
LIGHT属于TNF配体家族，并且可以通过疱疹病毒进入介质A型受体（HVEM，TNFRSF14），LTβR或与decoy受体DcR3结合而传导信号。它在脾细胞，活化PBL，CD8 +肿瘤浸润淋巴细胞，粒细胞和单核细胞中表达。LIGHT可以活化NF-κB以共刺激淋巴细胞的活化并诱导某些人类肿瘤细胞的凋亡。
BioGems鼠Light预包被ELISA试剂盒是一种固相免疫分析试剂，专门设计用于检测Light，并在96孔微孔板上预包被Light特异性小鼠单克隆抗体。 它含有NS0表达的重组Light。 检测抗体是对Light特异的生物素化山羊多克隆抗体。该试剂盒经过分析验证，是即用型试剂。
Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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93.7pg/ml-6000pg/ml
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Specificity:
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Natural and recombinant Murine Light
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Uniprot:
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Q9QYH9
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components组份
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Description
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Quantity
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Volume
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Anti-Murine Light Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Murine Light Standard
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2
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10 ng vial
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Murine Light Biotinylated antibody (100x)
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1
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130 µL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 µL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility可重复性
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|
Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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120
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501
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2100
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144.5
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649
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1750
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Standard Deviation
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7.56
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28.55
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102.9
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10.54
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50.62
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96.25
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Consistency (%CV)
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6.3
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5.7
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4.9
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7.3
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7.8
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5.5
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