磺化和非磺化染料都可用于标记生物分子例如DNA和蛋白质。染料之间的差异是它们的溶解性：磺基染料是水溶性的，并且它们不使用有机共溶剂用于在水性环境中的标记。它们不易于在水中聚集。

There are two varieties of cyanine dyes: non-sulfonated cyanines, and sulfonated cyanines. For many applications they are interchangeable, because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used for the labeling of biomolecules such as DNA and proteins. The difference between the dyes is their solubility: sulfo- dyes are water-soluble, and they do not use of organic co-solvent for the labeling in aqueous environment. They are less prone to aggregation in water.

• Sulfo-Cyanine3 carboxylic acid
• Sulfo-Cyanine5 carboxylic acid
• Sulfo-Cyanine5.5 carboxylic acid
• Sulfo-Cyanine7 carboxylic acid
• Sulfo-Cyanine7.5 carboxylic acid

无活性的磺酸基-Cy5羧酸，水溶性染料。此染料是高水溶性染料 用于分子标记应该选择具有活性的磺酸基-Cy5 NHS酯

分子结构：

一般性质： 外观：深蓝色粉末 分子量：664.76 分子式：C32H37N2NaO8S2 溶解性：易溶于水，DMSO,DMF，在多数有机溶剂难溶 质控：NMR 1H（95%）和13C,TLC

光谱性质： 最大激发波长：646nm 在最大激发波长下的消光系数：271000Lmol-1cm-1 最大发射波长：662nm 荧光量子产率：0.2

General properties

磺酸基-Cy7羧酸是无活性易溶于水的近红外染料； 此试剂不能用于标记分子，它有较高的水溶性，可改善近红外光谱范围的量子产率,并具有很高的消光系数。

分子结构：

一般性质： 外观：深绿色粉末 分子量：730.87 分子式：C37h33N2NaO8S2 溶解性：易溶于水，DMF,DMSO 质控：HPLC（95%），NMR 1H 和13C

光谱性质： 最大激发波长：740nm 在最大激发波长下的消光系数：240600Lmol-1cm-1 最大发射波长：773nm

General properties

Sulfo-Cyanine7 carboxylic acid is non-reactive water soluble near infrared dye.

The reagent is useful as a fluorescent marker in NIR range when attachment to other molecules is not desired. It has high hydrophilicity and aqueous solubility, improved quantum yield in NIR range, and very high molar extinction coefficient.

This reagent contains free carboxylic acid function. Pre-activated activated ester – sulfo-Cyanine7 NHS ester is also available.