品名：Recombinant Human BMP-8a Protein

规格：10ug

保存：-20°C~ -70 °C

说明书下载：Recombinant Human BMP-8a Protein

Purity： >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level： <0.10 EU per 1 μg of the protein by the LAL method.

Activity： Measured by its binding ability in a functional ELISA. When Recombinant Human BMP‑8a is present at 1 μg/mL,

the concentration of recombinant human BMPR‑1A Fc Chimera that produces 50% of the optimal binding response is approximately 1.5‑6 μg/mL.

Source： E. coli-derived Ala264-His402 & Val265-His402

Accession #： AAP74559

N-terminal Sequence Analysis： Ala264 & Val265

Predicted Molecular Mass： 15.8 (monomer) kDa

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Formulation： Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.

Reconstitution： Reconstitute at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Shipping： The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage： Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.

1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-8a

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.

There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy‑terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).