货品编号: R&D.455-MC-010 品牌:R&D systems
品名:Recombinant Mouse SCF Protein
规格:10ug
保存:-20°C~ -70 °C
说明书下载:Recombinant Mouse SCF Protein
Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Level:<0.10 EU per 1 μg of the protein by the LAL method.
Activity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura,
T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is typically 2.5-10 ng/mL
Source:E. coli-derived Lys26-Ala189, with an N-terminal Met
Accession #: #Q78ED8 N-terminal Sequence Analysis: Met
Predicted Molecular Mass: 18.6 kDa
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
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