货品编号： signalchem.b13-10g-05

品牌： signalchem

品名： BRSK1, Active

规格：5 ug

Product Details

Description:Recombinant full-length mouse BRSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Species:Mouse

Tag:GST tag

Expression System:Sf9 insect cells using baculovirus

Sequence:Full Length

Genbank Number:NM_001003920

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping : Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and

store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications : Kinase Assay, Western Blot

Molecular Weight : ~118 kDa

Gene Aliases :Gm1100; MGC99905; SAD-B; SADB

Scientific Background :BRSK1 is serine/threonine kinases 1 which required for presynaptic differentiation in Caenorhabditis elegans that are

needed for neuronal polarization (1). BRSK1 is highly expressed in all specific adult brain regions followed by fetal brain and adult spinal cord.

It is also expressed in adult heart, pancreas, testis, ovary, lung, and kidney, and in fetal liver (2).

References : 1. Kishi, M. et.al: Mammalian SAD kinases are required for neuronal polarization. Science 307: 929-932, 2005. 2. Nagase, T.et.al: Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from

brain which code for large proteins in vitro. DNA Res. 8: 85-95, 2001.

Formulation :50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.