货品编号： signalchem.c12-10h-05

品牌： signalchem.c12-10h-05

品名： signalchem.c12-10h-05

规格： signalchem.c12-10h-05

Product Details

Description:Recombinant full-length human CAMK2beta was expressed by baculovirus in Sf9 cells using an N-terminal His tag.

Species:Human

Tag:HIS tag

Expression System:Sf9 insect cells using baculovirus

Sequence:Full Length

Genbank Number: NM_172081

Specific Activity :Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.

Purity :Sample Purity Data. For specific information on a given lot, see related technical data sheet.

Storage, Stability and Shipping :Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and

store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Applications :Kinase Assay, Western Blot

Molecular Weight : ~58 kDa

Gene Aliases :CAMKB, CAM2, CAMK2, MGC29528

Scientific Background :CAMK2β belongs to the serine/threonine protein kinase family and to the type II multifunctional Ca(2+)/calmodulin-dependent

protein kinase subfamily. CAMK2β showed wide tissue and cell distribution, and one of CAMK2β variant predominated in adult brain (1).

The ratio of CAMK2α and CAMK2β protein levels were inversely related during activity in hippocampal neurons (2). CAMK2β is a prominent kinase in

the central nervous system and may function in long-term potentiation and neurotransmitter release.

References : 1. Tombes, R. M. et al: Identification of novel human tumor cell-specific CaMK-II variants. Biochim. Biophys. Acta 1355: 281-292, 1997. 2.Thiagarajan, T. C. Et al: Alpha- and beta-CaMKII: inverse regulation by neuronal activity and opposing effects on synaptic strength.

Neuron 36: 1103-1114, 2002.

Formulation :50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 2mM DTT, 25% glycerol.