货品编号： Thermo.A-21145

品牌： Thermo

品名： Goat anti-Mouse IgG2b Cross-Adsorbed Secondary Antibody

规格： 250 µL

产品描述

Flow cytometry analysis of Goat anti-Mouse IgG2b Secondary Antibody, Alexa Fluor 594 (Product # A21145)

was performed using Jurkat cells stained with E2F1 Mouse Monoclonal Antibody (Product # 321400). Cells

were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and

blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with E2F1 antibody or with mouse

isotype control at 3-5 ug/million cells in 2.5% BSA and incubated for 2 hours at room temperature. The cells were

then labeled with Goat anti-Mouse IgG2b Secondary Antibody, Alexa Fluor 594 (Product # A21145) at a dilution of

1:500 for 1 hour at room temperature.

Background/Target Information Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins

and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility

enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through

signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies

are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be

further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate

highly specific reagents.