货品编号： Thermofis.FD0454

品牌： Thermofis

品名： FastDigest Esp3I

规格： 20 reactions

产品描述

Thermo Scientific FastDigest Esp3I restriction enzyme recognizes CGTCTC(1/5)^ site and cuts best at 37°C in 5–15 minutes using

universal FastDigest Buffer. Isoschizomers: BsmBI, BstGZ53I, Esp16I, Esp23I.

Thermo Scientific FastDigest Esp3I is one of an advanced line of fast restriction enzymes that are all 100% active in the universal

FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change

or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and

incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase,

alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications

can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction

products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available

Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis

Note: FastDigest Esp3I is supplied in a storage buffer containing 7mM 2-mercaptoethanol.

The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the

colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration

measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.