货品编号： Thermofis.FD0434

品牌： Thermofis

品名： FastDigest ScaI

规格： 100 reactions

产品描述

Thermo Scientific FastDigest ScaI restriction enzyme recognizes AGT^ACT site and cuts best at 37°C in 5–15 minutes using universal

FastDigest Buffer. Isoschizomers: AssI, BmcAI, ZrmI.

Thermo Scientific FastDigest ScaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal

FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change

or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and

incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase,

alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications

can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction

products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available

Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as

the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration

measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.