货品编号: Fermentas.FD0694
品牌: Fermentas
品名: FASTDIGEST XHOI EA
规格: 400 reactions
产品描述
Thermo Scientific FastDigest XhoI restriction enzyme recognizes C^TCGAG site and cuts best at 37°C in 5–15 minutes
using universal FastDigest Buffer. Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI.
Thermo Scientific FastDigest XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the
universal FastDigest and FastDigest Green reaction buffers.
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for
buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme
activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes,
such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest
Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.
For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of
digestion reaction products on gels.
Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.
Features • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available
Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis
Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as
the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration
measurements in UV light) the colorless FastDigest Buffer is recommended.
For methylation sensitivity, refer to product specifications.
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