货品编号： Fermentas.FD0303

品牌： Fermentas

品名： FASTDIGEST ECO32I EA

规格： 200 reactions

产品描述

Thermo Scientific FastDigest Eco32I restriction enzyme recognizes GAT^ATC site and cuts best at 37°C in 5–15 minutes

using universal FastDigest Buffer. Isoschizomers: EcoRV, EcoRV.

Thermo Scientific FastDigest Eco32I is one of an advanced line of fast restriction enzymes that are all 100% active in the

universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for

buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme

activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes,

such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest

Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of

digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.

Features • 100% activity of all FastDigest enzymes in the universal buffer • 100% buffer compatibility with downstream applications • Complete digestion in 5–15 minutes • Direct loading on gels • No star activity • 176 FastDigest enzymes available

Applications • Molecular cloning • Restriction site mapping • Genotyping • Southern Blot • Restriction fragment length polymorphism (RFLP) • SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications

as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration

measurements in UV light) the colorless FastDigest Buffer is recommended.