货品编号: Invitrogen.11966-025
品牌: Invitrogen
品名: DMEM, no glucose
规格: 500ml
产品描述
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.
Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines
such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right
formulation using the media selector tool. This DMEM is modified as follows:
With Without • L-glutamine • Glucose • Phenol Red • Sodium Pyruvate • HEPES
The complete formulation is available.
Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal
Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels,
with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly
with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2
environment to maintain physiological pH.
cGMP manufacturing and quality system For supply chain continuity, we manufacture DMEM at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are
compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
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