货品编号： Thermo.22400-089

品牌： Thermo

品名： RPMI 1640 Medium, HEPES

规格： 500 mL/瓶

产品描述：

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.

Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells,

including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium

modifications for a range of cell culture applications. Find the right formulation using the media selector tool. This RPMI is modified as follows:

With Without • L-glutamine • HEPES • Phenol Red The complete formulation is available.

Using RPMI RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations

of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential

Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation,

commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and

therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation

of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a

letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with

the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical

RPMI 1640 product made in our Scotland facility (52400-025). This facility is registered with the FDA as a medical device manufacturer

and is certified to the ISO 13485 standard.