货品编号： Invitrogen.34580

品牌： Invitrogen

品名： SuperSignal™ West Pico PLUS Chemiluminescent Substrate

规格： 500 mL

产品描述

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL)

horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP

conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures

to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes,

blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.

Features of SuperSignal West Pico PLUS Chemiluminescent Substrate:

• Picogram to femtogram sensitivity—detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or

PVDF membrane • High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light

output under optimal conditions • Stable reagent—8-hour working solution stability; 1-year kit stability at room temperature • Economical—optimized for dilute antibody concentrations:--0.2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock)--10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock) • Exceptional robustness—provides high performance outside of the recommended antibody dilution ranges, including the most common

1:5K to 1:10K secondary antibody dilutions of a 1 mg/mL stock solution