货品编号: Invitrogen.F548L 品牌: Invitrogen 品名: Phusion Flahh High-Fidelity PCR Master Mix 规格: 500 reactions 储存条件: -20°C
产品描述
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix
preserves the fidelity and the yield in the reaction when using extremely short PCR protocols. Additionally, the user only needs to
add template and primers minimizing the number of pipetting steps. The unique master mix composition permits usage of extremely short cycling protocols with both low and high complexity DNA
templates - 15 seconds per kilobase or less. The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading
DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.
Features • Extreme speed: extension times of 15 s/kb or less • Hot start modification allowing “zero-time reactivation” • Accuracy: proofreading DNA polymerase with a fidelity of 25X Taq • High yields in reduced time
Applications • Fast PCR • High-fidelity PCR • Difficult (GC-rich) templates • Template generation for sequencing • Multiplex PCR • Long-range PCR • Cloning • Mutagenesis • Microarray
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