货品编号： Invitrogen.20217 品牌： Invitrogen 品名： EZ-Link NHS-Biotin 规格： 100mg/瓶

产品描述：

Thermo Scientific EZ-Link NHS-Biotin is the simplest NHS-ester activated biotinylation reagent for labeling primary amines

(e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.

Features of EZ-Link NHS-Biotin:

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins • Membrane-permeable—can be used to label inside cells (intracellular) • Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides • Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved • Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers • Very short—spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only

NHS-Biotin is N-hydroxysuccinimidobiotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and

efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. Differing only in their

spacer arm lengths, the three NHS-ester reagents offer researchers the possibility of optimizing labeling and detection experiments

where steric hindrance of biotin binding is an important factor. Because they are uncharged and contain simple alkyl-chain spacer

arms, these biotin compounds are membrane-permeable and useful for intracellular labeling.