货品编号： Invitrogen.16600082 品牌： Invitrogen 品名： McCoy's 5A (Modified) Medium 规格： 500 mL

产品描述：

McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of

primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of

primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other

tissues.

This McCoy's 5A is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • HEPES • Bacto-peptone • Phenol Red

The complete formulation is available.

Using McCoy's 5A (modified) Medium Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike

other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose.

This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A

(modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A

(modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2

environment to maintain physiological pH.