货品编号： Invitrogen.21312 品牌： Invitrogen 品名： EZ-Link™ NHS-PEG12-Biotin 规格： 25 mg

产品描述：

Thermo Scientific EZ-Link NHS-PEG12-Biotin is very long, pegylated biotinylation reagent for simple and efficient biotin labeling

of antibodies, proteins and other primary amine-containing macromolecules.

Features of EZ-Link NHS-PEG12-Biotin:

• Protein labeling—biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins • Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides • Pegylated—spacer arm contains a hydrophilic, 12-unit, polyethylene glycol (PEG) group • Enhances solubility—pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated

antibodies stored in solution • Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved • Very long reach—spacer arm (total length added to target) is 56 angstroms; this reduces steric hindrance when binding to avidin molecules

NHS-PEG12-Biotin is a very long (56.0Å), pegylated, water-soluble, NHS-ester biotinylation reagent to label amines and maximize

solubility of antibodies and other proteins. The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine

and N-terminal amino groups at pH 7-9 to form stable amide bonds. The hydrophilic polyethylene glycol (PEG) spacer arm imparts

water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG

spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance for binding to avidin molecules.