Sigma.D8417-5MG 4′,6-Diamidino-2-phenylindole dihydrochloride Sigma-Aldrich


货品编号: Sigma.D8417-5MG 品牌: Sigma 品名: 4′,6-Diamidino-2-phenylindole dihydrochloride 规格:5MG

产品描述

Biochem/physiol Actions Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially

to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

Caution Protect from light.

包装 1, 5, 10 mg in glass bottle

Application DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of

DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes

in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific

detection method for mycoplasma.

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