货品编号： sigma.DN25-100MG 品牌： sigma 品名： Deoxyribonuclease I脱氧核糖核酸酶 I 来源于牛胰腺 规格： 100mg 产品描述： Analysis Note Protein determined by biuret. Application 用于从蛋白质样品中除去 DNA。 DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture.[2] It has also been used along with trypsin for the preparation of single cell suspension from rat testes.[3] Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis. 包装 1, 5 g in poly bottle 10, 100 mg in poly bottle Preparation Note 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration. Unit Definition One Kunitz unit will produce a change in A260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III, as substrate. Physical form Crude preparation, contains calcium chloride