Lumiprobe蛋白质巯基标记用活性染料

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Cy3马来酰胺： Cyanine3 maleimide

巯基活性Cy3染料，可以将Cy3荧光团结合到蛋白和多肽的巯基上。

分子结构：

一般性质： 外观：红色粉末 分子量：615.2 分子式：C36H43ClN4O3 溶解性：溶于有机溶剂（DMF，DMSO，二氯甲烷），不溶于水 质控：NMR 1H（95%）和13C,TLC，功能测试

光谱性质： 最大激发波长：555nm 在最大激发波长下的消光系数：150000Lmol-1cm-1 最大发射波长:570nm 荧光量子产率：0.31

General properties

Appearance: red powder Molecular weight: 615.20 Molecular formula: C₃₆H₄₃ClN₄O₃ Solubility: soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water Quality control: NMR ¹H, HPLC-MS (95%) Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Thiol mono-reactive Cyanine3 dye. This reagent can be used to attach Cyanine3 fluorophore (an analog of Cy3®) to proteins and peptides containing cysteine residues, as well as to other thiolated molecules (such as thiol-containing oligonucleotides).

Cystines should be reduced with TCEP (tris-carboxyethylphosphine) or other appropriate reductant prior to the labeling.

Labeling with Cyanine3 maleimide is selective, and efficient.

We recommend using water-soluble Sulfo-Cyanine3 maleimide for the labeling of antibodies and other sensitive proteins.