货品编号： Thermo.P36013 品牌： Thermo 品名：pHrodo iFL Green STP Ester (amine-reactive) 规格：3x100μg

内容及存储： Store in freezer (-5 to -30°C) and protect from light.

产品描述：

The amine-reactive pH-sensitive pHrodo iFL Green STP Ester dye is an improved version of pHrodo Green dye

optimized for the creation of bioconjugates to be used in the study of antibody internalization, endocytosis, and

phagocytosis. pHrodo iFL Green dye dramatically increases fluorescence as the pH of its surroundings become

more acidic. It is more soluble than the original pHrodo Green dye, making it more useful for the labeling of antibodies

that may otherwise precipitate out of solution during conjugation.

• Use pH-sensitive pHrodo iFL Green STP Ester to make pH-sensitive bioconjugates of your choice • Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps

or quenchers • Multiplex with red fluorescent markers such as RFP, pHrodo iFL Red STP, and many others

The increase in fluorescence of pHrodo iFL Green dye as the pH changes from basic to acidic correlates with the

acidification of intracellular vesicles, making it a valuable tool for the study of endocytosis or phagocytosis and their

regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo iFL Green dye make it useful

for multi-color experiments. pHrodo iFL Green dye can be detected using most standard green-fluorescent filter sets and

has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy, and high content

screening (HCS). The lack of fluorescence of pHrodo iFL Green dye in a typical extracellular environment eliminates the

need for wash steps or quencher dyes in the experimental workflow.