货品编号： Thermo.P36974 品牌：Thermo 品名：ProLong Live Antifade Reagent, for live cell imaging 规格：5x1mL

产品描述：

ProLong® Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging.

When dyes are illuminated, they can degrade (usually permanently), reducing the sample’s fluorescent intensity and leading

to the creation of free radical singlet oxygen, an element that can further degrade neighboring dye molecules. ProLong® Live

Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal

low. ProLong® Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up

to 48 hours.

Features of ProLong® Live Antifade Reagent include: • Photobleach protection in live cell experiments, for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker®,

and CellTracker®dyes • Little-to-no effect on cellular viability, proliferation, or other cellular functions • Easy to use, just add to the imaging solution or complete cell culture media or buffer, incubate, and image

ProLong® Live Antifade Reagent contains enzymes from the plasma membrane of naturally occurring E. coli. These enzymes

metabolize the elements that can cause photobleaching. They work in the extracellular space and are not known to enter cells, so

intracellular functions are minimally affected by ProLong® Live Antifade Reagent. As shown in figure below, cells grown in the

presence of ProLong® Live Antifade Reagent display minimal effects on cellular viability or proliferation. ProLong® Live Antifade

Reagent provides photobleach protection for most fluorophores frequently used in live cell fluorescent imaging, such as GFP, RFP,

Hoechst 33342, MitoTracker®, LysoTracker® and CellTracker® dyes. ProLong® Live Antifade Reagent can be added to any cell

culture media or buffer that is suitable for fluorescent imaging.