货品编号： Thermo.A-21207 品牌：Thermo 品名：Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 规格： 500ul 产品描述：

Product Specific Information To minimize cross-reactivity, these donkey anti-rabbit IgG whole antibodies have been affinity-purified and show minimum

cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-

adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less

background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum

proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column,

and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining

experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent

staining experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 594 dye is a bright, red-

fluorescent dye with excitation ideally suited to the 594 nm laser line. For stable signal generation in imaging and flow cytometry,

Alexa Fluor 594 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability

allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 594 dye molecules can be attached to

proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree

of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the

certificate of analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to

the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific

background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow

cytometry applications.