货品编号： Sigma.G1393-100ML 品牌：Sigma 品名：液体明胶 规格：100ML 产品描述：

Components Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen.

Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived

from acid-cured tissue. Type B is derived from lime-cured tissue.

Application This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration

does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to

PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of

media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of

active biomolecules and in generation of scaffolds for tissue engineering applications. It has also been used to study long-chain

fatty acid-induced changes in gene expression in neonatal cardiac monocytes and to study to test mobilization of capillary

endothelium in vitro induced by effectors of angiogenesis in vivo.

Caution Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at

100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases

and ammonia.

Preparation Note This product is derived lime-cured tissue bovine skin, and is a 2% solution in water.