Thermo.11-5321-81 Anti-Mouse MHC Class II (I-A/I-E) FITC 50ugThermo


货品编号: Thermo.11-5321-81 品牌:Thermo 品名:Anti-Mouse MHC Class II (I-A/I-E) FITC 规格:50ug

产品描述:

Product Specific Information Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II,

both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects

a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes

from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes.

The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k,

or H-2 s haplotypes.

Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis.

Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can

be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample

in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is

recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

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