货品编号: Thermo.11-5931-81 品牌:Thermo 品名:Anti-Mouse Ly-6G (Gr-1) FITC 规格:50ug
产品描述:
Product Specific Information Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the
myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally
regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow
development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker
for these populations.
eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells
that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G
(cat. 9668) be used when looking at Ly-6G specific targets.
Applications Reported: The RB6-8C5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and
splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that
will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to
10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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