货品编号: Thermo.21063029 品牌:Invitrogen 品名:DMEM, high glucose, HEPES, no phenol red 规格:500ml/瓶
产品描述: DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many
different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells,
HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety
of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector
tool.
This DMEM is modified as follows: With Without • High Glucose • Sodium Pyruvate • L-glutamine • Phenol Red • HEPES
The complete formulation is available.
Using DMEM DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original
Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is
often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors.
Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium
bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA
as a medical device manufacturer and is certified to ISO 13485 standards.