产品描述： DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the

growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts,

human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture

applications. Find the right formulation using the media selector tool.

This DMEM/F-12 is modified as follows: With • L-glutamine • HEPES • Phenol Red

The complete formulation is available.

Using DMEM/F-12 DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose,

amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors.

Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium

bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system DMEM/F-12 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA

as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM/F-12

product made in our Scotland facility (31330-038). This facility is registered with the FDA as a medical device manufacturer and is certified

to the ISO 13485 standard.