货品编号： Invitrogen.A31570 品牌：Invitrogen 品名：Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 规格： 500ul/支

产品描述： Product Specific Information To minimize cross-reactivity, these donkey anti-mouse IgG whole antibodies have been affinity-purified and show minimum

cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins. Cross-

adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less

background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins

from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the

highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments

(e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining

experiments where there may be the presence of endogenous immunoglobulins.

Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 555 dye is a bright, orange-

fluorescent dye with excitation ideally suited to the 555 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa

Fluor 555 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow

detection of low-abundance biological structures with great sensitivity. Alexa Fluor 555 dye molecules can be attached to proteins at

high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling

for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of

analysis for each product lot.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to

the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific

background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.

For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow

cytometry applications.