货品编号： Invitrogen.A32728 品牌：Invitrogen 品名：Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 规格：1mg 储存条件： 4° C, store in dark

产品描述：

Product Specific Information To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG,

goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to

increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution

is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the

nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further

passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these

extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other

primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant

to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing

nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be

determined empirically.