货品编号： thermo.a39269 品牌：thermo 品名：Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weigh™ Format 规格：10x2mg 储存条件： Store at 4°C.

产品描述：

Thermo Scientific Pierce Sulfo-NHS, No-Weigh Format is a chemical modification reagent for converting carboxyl

groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.

Features of Sulfo-NHS:

• Efficiency of EDC-mediated coupling is increased in the presence of Sulfo-NHS • Amine-reactive NHS esters or Sulfo-NHS esters can be made with any carboxyl-containing molecule • Sulfo-NHS derivatives are usually directly water-soluble (can be added directly to physiologic buffers) and membrane-

impermable (can be used for cell surface labeling) • High-purity, crystalline Sulfo-NHS can be used to create high-quality activated derivatives

Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving

activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by

mixing the Sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC).

The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes,

biotin affinity tags and pegylation compounds.

Applications: • Improve efficiency of EDC coupling reactions • Convert carboxyls to amine-reactive Sulfo-NHS esters • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently • Activate nanoparticles with amine-reactive Sulfo-NHS esters