biorad.3450129 3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl Bio-Rad


货品编号: biorad.3450129 品牌:biorad 品名:3–8% Criterion™ XT Tris-Acetate Protein Gel, 12+2 well, 45 µl

产品描述: Choose this versatile midi Criterion XT Tris-Acetate Gel for denaturing or nondenaturing PAGE. The gels are made without

SDS, which allow the sample buffer and running buffer to determine the separation mechanism.

For native PAGE, use Native Sample and Tris/Glycine Native Running Buffers For separation by molecular weight, use XT Sample and XT Tricine Running Buffers Neutral pH (pH 7.0) delays acrylamide hydrolysis as compared to traditional Laemmli systems 8-month shelf life Separation range is ~40–400 kD under denaturing conditions Two wells available for markers or other references Can be loaded with a multichannel pipet

Under denaturing conditions, with the SDS-containing Tricine running buffer, the discontinuous acetate and Tricine ion fronts

form moving boundaries to stack and then separate proteins by molecular weight. During native electrophoresis, where proteins

are separated by mass-to-charge ratio, discontinuous acetate and glycine ion fronts form moving boundaries to stack and separate

proteins by both size and charge.

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