货品编号： cayman.701040 品牌：cayman 品名：MitoCheck® Citrate Synthase Activity Assay Kit 规格：96T/盒
产品描述： Features Measure citrate synthase activity in isolated mitochondria, tissue, or cell homogenate Assess mitochondrial content Suitable for high-throughput screening Plate-based colorimetric measurement (412 nm) MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010) available to aid in mitochondrial tissue isolation
The condensation of the dicarboxylate oxaloacetate and acetyl CoA to the tricarboxylate citrate is catalyzed by citrate synthase. It is
within this reaction that carbon molecules (as acetyl CoA) obtained from pyruvate oxidation are fed into the tricarboxylic acid (TCA or
citric acid) cycle. As a mitochondrial enzyme, citrate synthase is commonly used as a normalization factor for mitochondrial protein, but
can also be used as a biomarker for mitochondrial content in a tissue homogenate. Cayman's MitoCheck® Citrate Synthase Activity
Assay Kit allows for the simple and convenient determination of citrate synthase activity from isolated mitochondria, tissue, or cell
homogenates. This assay measures the production of SH-CoA by monitoring the absorbance of Citrate Synthase Developing Reagent
at 412 nm in a convenient 96-well format.
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